| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
18/03/2016 |
|
Data da última atualização: |
14/02/2018 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. |
|
Afiliação: |
JULIANA MARCOLINO GOMES, UEL; THIAGO J. NAKAYAMA, UFV; RAFAELA RIBEIRO REIS, UEL; FABIANA APARECIDA RODRIGUES; LILIANE MARCIA MERTZ HENNING, CNPSO; RENATA FUGANTI PAGLIARINI; HUGO BRUNO CORREA MOLINARI, CNPAE; FRANK HARMON, USDA; ALEXANDRE LIMA NEPOMUCENO, SRI. |
|
Título: |
Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. |
|
Páginas: |
PO 203 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Drought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans. MenosDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants ... Mostrar Tudo |
|
Thesagro: |
Gene; Resistência à seca; Soja. |
|
Thesaurus Nal: |
Soybeans; Water stress. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141414/1/Gene-expression-and-functional-...-2015.html
|
|
Marc: |
LEADER 02573nam a2200277 a 4500
001 2041429
005 2018-02-14
008 2015 bl uuuu u00u1 u #d
100 1 $aGOMES, J. M.
245 $aGene expression and functional analysis of soybean genes with diurnal oscillation during drought stress.$h[electronic resource]
260 $aIn: PLANT & ANIMAL GENOME XXIII, 2015, San Diego.$c2015
300 $aPO 203
520 $aDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans.
650 $aSoybeans
650 $aWater stress
650 $aGene
650 $aResistência à seca
650 $aSoja
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aRODRIGUES, F. A.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aMOLINARI, H. B. C.
700 1 $aHARMON, F.
700 1 $aNEPOMUCENO, A. L.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Semiárido. |
|
Data corrente: |
22/02/2013 |
|
Data da última atualização: |
09/01/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
FERNANDES JUNIOR, P. I.; LIMA, A. A. de; PASSOS, S. R.; GAVA, C. A. T.; OLIVEIRA, P. J. de; RUMJANEK, N. G.; XAVIER, G. R. |
|
Afiliação: |
PAULO IVAN FERNANDES JUNIOR, CPATSA; ANDRÉA APARECIDA DE LIMA, UFRRJ; SAMUEL RIBEIRO PASSOS, UFRRJ; CARLOS ALBERTO TUAO GAVA, CPATSA; PAULO JANSEN DE OLIVEIRA, UFRRJ; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB. |
|
Título: |
Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1604-1612, 2012. |
|
ISSN: |
1517-8382 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization |
|
Palavras-Chave: |
Biological nitrogen fixation; Diversidade fenotípica; FBN; Fixação biológica de nitrogênio; Rizóbio; Seleção de estirpe; Seleção de estirpes; Strain selection. |
|
Thesagro: |
Feijão; Feijão de Corda; Melhoramento Vegetal. |
|
Thesaurus NAL: |
biotechnology; Molecular genetics; Nitrogen fixation; Pigeon peas. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/78769/1/Paulo-Ivan-2012.pdf
|
|
Marc: |
LEADER 02518naa a2200385 a 4500
001 1950566
005 2023-01-09
008 2012 bl uuuu u00u1 u #d
022 $a1517-8382
100 1 $aFERNANDES JUNIOR, P. I.
245 $aPhenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.].
260 $c2012
520 $aThis study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization
650 $abiotechnology
650 $aMolecular genetics
650 $aNitrogen fixation
650 $aPigeon peas
650 $aFeijão
650 $aFeijão de Corda
650 $aMelhoramento Vegetal
653 $aBiological nitrogen fixation
653 $aDiversidade fenotípica
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aRizóbio
653 $aSeleção de estirpe
653 $aSeleção de estirpes
653 $aStrain selection
700 1 $aLIMA, A. A. de
700 1 $aPASSOS, S. R.
700 1 $aGAVA, C. A. T.
700 1 $aOLIVEIRA, P. J. de
700 1 $aRUMJANEK, N. G.
700 1 $aXAVIER, G. R.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1604-1612, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
